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These three specimens use recorded TEM images. Other training specimens are synthetic. Recorded images receive simulated display effects; digital enlargement adds no recorded detail. Their scale is estimated from the source bars, not a new instrument calibration.
MVLscope display adaptation revised 19 September 2026: cropping and resampling for navigation, with illustrative focus, astigmatism, contrast, illumination, noise and related effects. Annotated captures add credit and an approximate scale bar. This is the adaptation revision date, not the source image date. Simulated settings are not the original acquisition conditions.
Credit: Dartmouth Electron Microscope Facility, Dartmouth College, via Wikimedia Commons. Source image and author public-domain release.
The displayed crop excludes the source footer. Scale is estimated from its original 200 nm bar. The Commons description is dated 20 September 2007; this is not an independently verified acquisition date.
Credit: National Institute of Standards and Technology (NIST). Source comparison image · NIST image policy · NIST non-SRD terms and notices.
The adaptation uses the continuous-beam left panel, cropped above the original scale label; scale is estimated from its 5 nm bar. The image page is undated. Its accompanying NIST article was published on 24 February 2020.
NIST allows copying of unmarked web material with image acknowledgment. Its linked non-SRD policy covers reuse and adaptations, including foreign rights, and provides the complete conditions and disclaimers. The image is credited to NIST; it is not labelled CC0 or assigned a Creative Commons licence here. Credit does not imply endorsement of MVLscope or its simulated effects.
Credit: Louisa Howard, Dartmouth Electron Microscope Facility, via Wikimedia Commons. Source image and author public-domain release.
The displayed crop excludes the source footer. Scale is estimated from its original 50 nm bar; the short raster bar limits precision. The source does not state a creation date.
Source records checked 19 September 2026. The archived Dartmouth image index also records the grayscale collection's public-domain notice. The two biological files match the originals hosted by Commons. NIST file identity has not been independently compared with the remote bytes. Asset inventory and crop details.
Spreading a fixed current over more area lowers current density. Spot number and condenser aperture also affect current. The control called Brightness · C2 adjusts beam coverage here; it does not calculate the electron source's brightness.
Nominal dose is accumulated incident fluence: current × illuminated time ÷ (electron charge × area). Blanking and closed column valves stop it. Moving the viewing screen or a downstream beam stop does not protect the specimen. Simulation pause freezes the teaching clock.
The model uses a symmetric C2 spread around 0.50, an inverse spot-number current mapping, and aperture-opening area scaling for a uniform pupil. These are teaching assumptions; real lens transfer and spot numbering differ. At the default 0.32 / spot 3 / 100 µm aperture, the nominal reference is 0.085 nA over 2.3716 µm². These chosen values preserve the exercise timing; they are not measured TEM calibration data. Retracting C2 uses a full-opening reference of 180 µm.
Display gain is a fixed power of relative current density (exponent 0.35), capped at 1.8. Pixels may clip. Acquisition captures a frozen frame with illustrative noise and drift, not integrated detector counts. The analytical CTF preview is normalized and separate from image intensity.
Nominal dose assumes centred illumination. Aperture offsets visually clip the beam, but the counter is not a spatial map of dose on a moving specimen. The 3D rays, STEM dose, diffraction intensities and damage thresholds are schematic. Convergence angle and electron transport are not calculated.
These references support the principles; they do not validate this simulator or imply endorsement. Recorded-image credits are listed above and remain available in the specimen library, capture metadata and annotated captures.
The recording uses a synthetic aluminium alloy foil. Selected-area aperture sampling is not implemented; the acquisition records a model snapshot. The video is a teaching walkthrough, not a facility operating procedure.
